recombinant human cxcl16  (R&D Systems)

Journal: Clinical Cancer Research

Article Title: Redirecting B7-H3.CAR T Cells to Chemokines Expressed in Osteosarcoma Enhances Homing and Antitumor Activity in Preclinical Models

doi: 10.1158/1078-0432.CCR-23-3298

Figure Lengend Snippet: RNA-seq and multiplex analyses identify chemokines produced by primary OS samples. A, Expression of 28 chemokines by RNA-seq (FPKM shown; n = 85 OS samples); screen cutoff shown at 1 FPKM. B, Expression of 28 chemokines by protein secretion ( n = 8 patient samples); screen cutoff shown at 3 × 10 3 integrated pixel density. Green bars in A and B denote ligands deemed positive in both RNA and protein screens; error bars indicate SEM. C, Chemokine receptors (ligands in parentheses) CXCR2 (IL8), CXCR3 (CXCL10), CXCR6 (CXCL16), CCR7 (CCL21), and CCR8 (CCL18) as expressed endogenously and detected by flow cytometry on activated T cells ( n = 5 healthy donors; error bars indicate SEM). D, ELISA demonstrating detection of IL8 and CXCL16 in patient transport media specimens ( n = 6 patient tumor samples, 1 normal bone sample; error bars indicate SEM).

Article Snippet: At time point 0, the transwell was placed into a fresh V-shaped lower-chamber plate with 100 μL of RPMI supplemented with 1% FBS and 0, 10, or 30 ng/mL of recombinant human IL8 (R&D Systems) or recombinant human CXCL16 (R&D Systems).

Techniques: RNA Sequencing Assay, Multiplex Assay, Produced, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4 + macrophage infiltration

doi: 10.1038/s41467-024-51096-0

Figure Lengend Snippet: A Representative flow plots show the frequency of the CXCR6 + CD8 + GZMB + T cells (left) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. B Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells (left) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. C , D Representative flow plots show frequency of the CXCR6 + CD8 + GZMB + T cells ( C ) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells ( D ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. E , F Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells ( E ) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells ( F ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. G Representative images of 4NQO-induced HNSCC (upper) and corresponding HE staining(lower) in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 1 mm (upper), 100 μm (lower). H – K Representative images of KI67 ( H ) and Caspase-3 ( J ) IHC staining and quantitation of the percentage of KI67 + ( I ) and Caspase-3 + ( K ) cells in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 50 μm. Data are shown as mean ± SD (n = 8 mice). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. Source data and exact p values are provided as a Source Data file.

Article Snippet: In the co-culture system, the Moc1 cells were treated with different proportions of CD8 + T cells and PF4 + Macrophages, or with different concentrations of CXCL16 recombinant protein (HY-P700266, MCE).

Techniques: Comparison, Recombinant, Staining, Immunohistochemistry, Quantitation Assay